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T05023: Synthesis of phytoestrogen standards in labelled and unlabelled form

Wednesday 22 October 2003

This research project aims to produce analytical standards for the accurate measurement of phytoestrogens in food.

Study Duration : June 1998 to May 2002

Contractor : University of St Andrews

Background

The ability to accurately measure phytoestrogens in food as well as in samples of blood and urine is vital to be able to assess the human exposure and biological effects of these compounds. Analysis is critically dependent on the availability of pure labelled and unlabelled analytical standards for phytoestrogens and their metabolites. However, the range and availability of these compounds is limited. This project will develop methods for the preparation of analytical standards for phytoestrogens and their metabolites. This project will also extend and expand on the research performed in project T05001.

Research Approach

Labelled ( 13 C labels) and unlabelled phytoestrogens and their metabolites will be prepared using synthetic chemical methods for use in analytical studies.

Results and findings

This project has successfully produced a comprehensive set of 13 C-labelled phytoestrogen standards that have been used by other contractors in the T05 programme to undertake metabolic and analytical studies on phytoestrogens

A patent between the Agency and the University of St Andrews has been agreed to protect the synthetic methods used to make these materials, as well as their application in metabolic and analytical studies.

Dissemination information

The final report is available from the FSA Library and Information centre.
To obtain a copy, please contact the Enquiry Desk, Dr Elsie Widdowson Library and Information Services, Food Standards Agency ( tel: 020 7276 8181/8182 or email: library&info@foodstandards.gsi.gov.uk )

Publications:

M.F. Oldfield, L. Chen and N.P. Botting, (2004) The synthesis of [3,4,8- 13 C 3 ]daidzein, Tetrahedron, 60, 1887-1893.

N. Al-Maharik and N.P. Botting, (2004) A new short synthesis of coumestrol and its application for the synthesis of [6,6a,11a- 13 C 3 ]-coumestrol, Tetrahedron, 60, 1637-1642.

N. Al-Maharik and N.P. Botting, (2003) Synthesis of lupiwighteone via a para-Claisen-Cope rearrangement, Tetrahedron, 59, 4177-4181.

Contact : Email : science@foodstandards.gsi.gov.uk

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