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Q01071: Optimisation and validation of the quantitative microsatellite assays for premium rice varieties

Thursday 28 August 2003

This research project aims to develop a method that can differentiate pure-bred and hybrid Basmati rice and other long grain rice varieties.

Study Duration : August 2002 to April 2003

Contractor : Central Science Laboratories (CSL)

Background

Owing to its desirable characteristics, such as aroma and flavour, Basmati rice is sold in UK supermarkets at a premium price. This price incentive may lead to substitution or adulteration of Basmati rice with cheaper, lower quality varieties of rice by unscrupulous traders. Work at Nottingham University (Project Q01042) identified a number of microsatellite markers which are of use in distinguishing pure bred basmati, hybrid basmati and long grain rice types. These markers will need to be developed into a quantitative assay.

Research Approach

Project Q01071 aims to optimise and assess these markers for suitability for use in a quantitative assay. The assay will then be streamlined and the markers which have the most appropriate allelic pattern and provide the most consistent ability to differentiate between rice types will be identified. The markers will then be tested and validated using blind samples.

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